Bulk Handling Racks for Free-Floating Serial Sections, Especially Suitable for Nauta Staining

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Abstract
Staining racks, each containing 20 shallow compartments, were constructed. Sections were placed serially (one to each compartment) in the racks, and stacks of up to 9 racks were clamped. Thus, sections could be handled easily and kept in strict serial order, even in bulk. For Nauta staining, brains had to be gelatin embedded before sectioning. By storing sections in groups of 4 in ice-cube trays, evenly spaced series could be selected for placement in the racks. As many as 160 sections were taken together through all stages of the Nauta method, the timing of critical stages being controlled by taking 4 to 6 free-floating sections, together with the racks, through the various solutions.