Cloning of an α-glucosidase gene fromThermococcus hydrothermalisby functional complementation of aSaccharomyces cerevisiae mal11mutant strain
- 10 September 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 458 (2) , 188-192
- https://doi.org/10.1016/s0014-5793(99)01155-2
Abstract
α-Glucosidase is found in methanogenic and thermophilic archaea and also in eukaryotes and bacteria. The gene encoding the enzyme was cloned from Thermococcus hydrothermalis by complementation of a Saccharomyces cerevisiae deficiency maltase mutant strain. The gDNA clone isolated encodes an open reading frame corresponding to a protein of 242 amino acids. The protein shows 42% identity to a Pyrococcus horikoshii unknown ORF but no similarities were obtained with polysaccharidase sequences.Keywords
This publication has 17 references indexed in Scilit:
- Enzymes from Sulfolobus shibatae for the production of trehalose and glucose from starchExtremophiles, 1998
- Production of thermostable amylolytic enzymes by Thermococcus hydrothermalisBiotechnology Letters, 1998
- Thermococcus hydrothermalis sp. nov., a New Hyperthermophilic Archaeon Isolated from a Deep-Sea Hydrothermal VentInternational Journal of Systematic and Evolutionary Microbiology, 1997
- Properties and stabilization of an extracellular α-glucosidase from the extremely thermophilic archaebacteria Thermococcus strain AN 1: enzyme activity at 130°CBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1996
- Purification and characterization of a maltase from the extremely thermophilic crenarchaeote Sulfolobus solfataricusJournal of Bacteriology, 1995
- Classification of Someα-Glucosidases andα-Xylosidases on the Basis of Substrate SpecificityBioscience, Biotechnology, and Biochemistry, 1994
- Construction of glucose-repressible yeast expression vectorsGene, 1993
- Improved method for high efficiency transformation of intact yeast cellsNucleic Acids Research, 1992
- Purification and characterization of an alpha-glucosidase from a hyperthermophilic archaebacterium, Pyrococcus furiosus, exhibiting a temperature optimum of 105 to 115 degrees CJournal of Bacteriology, 1990
- Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of Escherichia coli by R-Factor DNAProceedings of the National Academy of Sciences, 1972