Structure of the extra‐membranous domain of the β‐subunit of (Na,K)‐ATPase revealed by the sequences of its tryptic peptides
Open Access
- 18 August 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 204 (2) , 297-301
- https://doi.org/10.1016/0014-5793(86)80832-8
Abstract
Membrane bound dog kidney (Na,K)‐ATPase was digested with trypsin. The peptides that were recovered in the supernatant were purified and sequenced. By comparing these results with the sequence of α‐ and human β‐subunits, the location of each of the peptides could be allotted. Both accessibility to trypsin and the facility of release into the water phase indicated that these peptides were derived from the exposed surface of the intact enzyme. The sequence, GXGXXG, reported in the Torpedo californica β‐subunit [(1986) FEBS Lett. 196, 315‐319] was likely a mere coincidence with the sequence of the dinucleotide‐binding site, since the last glycine was replaced by proline in the sequence of the dog β‐subunit. A disulfide bridge was found within a peptide derived from the β‐subunit. A possible model for the β‐subunit structure is proposed.Keywords
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