RECOVERY OF THERMALLY INJURED Saccharomyces cerevisiae: EFFECTS OF MEDIA AND STORAGE CONDITIONS

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Abstract
Resting cells of Saccharomyces cerevisiae Y25 were heated at 56°C for 0–5 mm. Viability and thermal injury were evaluated using various media. Delayed plating after storage in water at 22°C resulted in increased counts primarily due to repair of thermal injury. Repair of injured cells was prevented by storage at 4°C or in the presence of 2,4‐dinitrophenol, but was not affected by storage in the presence of actinomycin D, chloramphenicol, cycloheximide or hydroxyurea. Recovery of injured cells was inversely related to glucose concentration in autoclaved media. However, counts on a yeast nitrogen base‐glucose medium were considerably higher when glucose was filter‐sterilized rather than autoclaved.