New localizations of VH sequences by in situ hybridization with biotinylated probes
- 1 July 1990
- journal article
- research article
- Published by Wiley in Genes, Chromosomes and Cancer
- Vol. 2 (2) , 103-108
- https://doi.org/10.1002/gcc.2870020205
Abstract
The chromosomal localization of genes of three VH families (VH1–3) was performed using in situ hybridization with biotinylated probes. Significantly strong signals were observed on chromosome 14, band 14q32, and on bands 16p11 and 15q11, although less frequently. Signal intensity and frequency were more important on chromosome 14 with all three probes, and on chromosome 16 with the VH2 and VH3 probes, while chromosome 15 was more marked than 16 with the VH1 probe. The localization of VH genes on chromosomes other than 14 suggests that several genes of the VH family had been simultaneously translocated in evolution and that the newly localized VH sequences may be pseudogenes.This publication has 21 references indexed in Scilit:
- Detection of single-copy genes by nonisotopic in situ hybridization on human chromosomesHuman Genetics, 1989
- Transposed human immunoglobulin Vκ gene regions carry clusters of conserved sequence elementsGene, 1988
- A novel family of variable region genes of the human immunoglobulin heavy chainJournal of Molecular Biology, 1987
- Organization and evolution of variable region genes of the human immunoglobulin heavy chainJournal of Molecular Biology, 1986
- Dispersed human immunoglobulin κ light-chain genesNature, 1986
- Clustering of extensive somatic mutations in the variable region of an immunoglobulin heavy chain gene from a human B cell lymphomaCell, 1986
- Genes for immunoglobulin heavy chains and for α1-antitrypsin are localized to specific regions of chromosome 14qNature, 1982
- Human Immunoglobulin Heavy Chain Genes Map to a Region of Translocations in Malignant B LymphocytesScience, 1982
- A simple method of reducing the fading of immunofluorescence during microscopyJournal of Immunological Methods, 1981
- Pattern of chromosomal replication in synchronized lymphocytesHuman Genetics, 1980