USE OF MONOCLONAL-ANTIBODIES TO IDENTIFY SHARED IDIOTYPES ON ANTICARDIOLIPIN AND ANTI-DNA ANTIBODIES IN HUMAN-SERA

  • 1 October 1987
    • journal article
    • research article
    • Vol. 70  (1) , 18-25
Abstract
Using hybridoma technology we produced monoclonal antibodies (MoAb) to idiotypic determinants on human anti-cardiolipin antibodies purified from a patient with SLE. Hybridomas were screened by inhibition of cardiolipin binding activity of sera from patients with SLE. Seven hybridomas were selected, tow of which were studied extensively. Sera from a number of patients with SLE were found to share isiotypic determinants. This cross-reacting idiotype was not detectable on anti-cardiolipin antibodies in syphilis sera. The cross-reacting idiotype was present in sera with anti-ssDNA antibodies even though some of these sera had no anti-cardiolipin antibodies. We propose that these MoAb may recognize regulatory idiotype.