INVITRO CULTIVATION OF ANIMAL-INFECTIVE FORMS OF A WEST-AFRICAN TRYPANOSOMA-VIVAX STOCK

  • 1 January 1982
    • journal article
    • research article
    • Vol. 39  (2) , 135-141
Abstract
Animal-infective forms of a West African Trypanosoma vivax stock were grown in culture for 3 mo. using Minimum Essential Medium (MEM) with Earle''s salts, supplemented with 20% inactivated goat serum over fibroblast-like cell lines isolated from the embryo of Microtus montanus or of an East African Galla crossbred goat at 36.5.degree. C and in 4% CO2-96% air. The blood-stream trypanosomes used to initiate the culture had been isolated from an infected goat. The cultured organisms grown in this system could be subcultured, were infective for mammalian hosts, retained their morphological characteristics and virulence and could be readily established in Glossina morsitans centralis from goats injected with the cultured T. vivax.