Distribution of C 14 from Glucose- 1-C 14 in the Lipid Fractions of Debaryomyces hansenii

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Abstract
Debaryomyces hansenii cells were grown in a medium containing yeast extract, malt-extract, glucose, sodium chloride, and nutrient salts, to which glucose-1-C14 was added. The lipids extracted from the cells were fractionated by use of a single column packed with silicic acid. Of the total C14 added to the culture medium, the neutral lipid fractions contained 21.06% while the phospholipid portions contained only 0.89%. The highest amount of C14 among the neutral lipids was found in the fraction containing the hydrocarbons (11.64%). Among the phospholipids, the highest amount (0.66%) was found in phosphatidyl serine and phosphatidyl ethanolamine.