Effect of pertussis toxin on parathyroid hormone-stimulated cyclic AMP production in cultured kidney cells
Open Access
- 1 December 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Bone and Mineral Research
- Vol. 3 (6) , 605-609
- https://doi.org/10.1002/jbmr.5650030605
Abstract
The effect of pertussis toxin, which inactivates the guanine nucleotide binding regulatory proteins Gi and Go on cAMP production in response to parathyroid hormone PGE2 or forskolin, was examined in confluent opossum kidney (OK) cells. This effect was compared with that caused by dexamethasone. The response to PTH was increased in cells preincubated with either agent. The effect of pertussis toxin was selective for PTH, since cAMP production in response to neither PGE2 nor forskolin was increased. In contrast, the response to forskolin was enhanced in dexamethasone-treated cells. These results indicate that both stimulatory and inhibitory guanine nucleotides binding regulatory proteins modulate PTH-induced cAMP producion in OK cells. Moreover, pertussis toxin and dexamethasone appear to affect different levels of the PTH-receptor-adenylate cyclase complex.Keywords
Funding Information
- Swiss National Science Foundation ((3.954.0.85))
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