On the Measurement of Dead Time in Micellar Liquid Chromatography

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Abstract
Modelling of the retention of solutes in micellar liquid chromatography allows the optimization of the resolution of a mixture of solutes and the determination of physico-chemical retention parameters. Both tasks imply the calculation of capacity factors, which are severely affected by the value of dead time. However, the determination of the dead time is not easy when a micellar mobile phase is used owing to the wide and variable perturbations that appear at the heads of the chromatograms. Four different criteria of determination of a reference time in the chromatograms are proposed and compared. The criteria are applied to mobile phases containing a varying concentration of surfactant and modifier, in order to observe the dependence of the reference time with the mobile phase composition. The study of the influence of the errors in the dead time on the modelling of the retention indicated that an accurate dead time is necessary to calculate retention parameters, but an excellent prediction of retention times can be achieved with a wide range of values of dead time.

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