Clones of Human Cytotoxic T Lymphocytes Derived from an Allosensitized Individual: HLA Specificity and Cell Surface Markers

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Abstract
By planned immunization of a volunteer, 2 stable (.gtoreq. 6 mo.), specific, alloreactive cytolytic T-cell clones were established from his peripheral blood lymphocytes. One clone reacts with all serologically defined HLA-Cw3 cells from our panel; the other defines a split within the serological HLA-B40 specificity. The 2 cytotoxic clones are surface membrane Ig-negative; E[sheep erythrocyte]-rosette positive; erythrocyte-antibody (EA) and erythrocyte-antibody-complement (EAC) rosette-negative; HLA-A-, -B- and -C-positive; and HLA-DR- or Ia-like-positive. They present very similar patterns of iodinated cell surface molecules as analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis, contrasting with that of an Epstein-Barr virus cell line derived from the same donor.