Studies on the reconstitution of Macromomycin and Auromomycin from the chromophore and protein moieties.

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Abstract
The chromophores extracted from macromomycin (MCR) and auromomycin (AUR) with methanol had identical UV absorption spectra, antibacterial spectra analytical profiles in high pressure liquid chromatography. The chromophore content of AUR was about 8 times higher than that of MCR. MCR reconstituted from the chromophore and protein function was identical with native MCR by Sephadex G-50 chromatography, UV absorption spectrum and antibacterial spectrum. The antibacterial activity of MCR and AUR was due to the chromophore; the protein moiety had no activity. The protein moiety enhanced the activity of the chromophore against gram-positive bacteria, while it suppressed the activity against gram-negative organisms. It also protected the chromophore from heat inactivation.