An enzyme‐linked immunosorbent assay for heat‐shock protein 70 in plants
- 1 January 1991
- journal article
- research article
- Published by Taylor & Francis in Food and Agricultural Immunology
- Vol. 3 (1) , 29-36
- https://doi.org/10.1080/09540109109354727
Abstract
Procedures are described for the production of antibodies against heat‐shock protein 70 (HSP70) from plants and for the development of an enzyme‐linked immunosorbent assay (ELISA) for the antigen in crude plant extracts. Competitive and two‐site sandwich variants of the ELISA are compared. Results are presented for a preliminary analysis of HSP70 levels in control and heat‐shocked mung bean shoots.Keywords
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