Potential influence of the first PCR cycles in real-time comparative gene quantifications
Open Access
- 1 August 2004
- journal article
- research article
- Published by Taylor & Francis in BioTechniques
- Vol. 37 (2) , 246-253
- https://doi.org/10.2144/04372rr01
Abstract
There is an underlying assumption in real-time PCR that the amplification efficiency is equal from the first cycles until a signal can be detected. In this study, we evaluated this assumption by an...Keywords
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