ACTIVITY OF SYNGENEIC COMPLEMENT FOR REVEALING ANTIBODY-INDUCED CYTOTOXICITY AGAINST A RAT HEPATOMA

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Abstract
The cytotoxicity of tumor-bearer serum against a transplanted aminoazodye-induced rat hepatoma was revealed using normal rat serum as the source of complement [C] as assessed using a short-term 51Cr release test. The tumor-bearing rat was not deficient in a functional C system and direct cytolysis against hepatoma cells was demonstrated following admixture of tumor cells and serum with no additional C. No tumor growth was observed in animals receiving s.c. transfer of cells treated with tumor-bearer serum, although when heat-inactivated (56.degree. C for 60 min) or normal sera were used, there was no modification of tumor development. The tumor-bearing animal apparently contains antibodies and C sufficient for tumor lysis, although the full contribution of serum-mediated cytotoxicity in imposing immunological constraints upon tumor growth remains to be elucidated.