Indirect Fluorescent Visualization Of Aliphatic Amines

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Abstract
2-Amino-5-methylbenzenesulfonic acid has been investigated as an indirect fluorescent visualization reagent in reversed phase ion pair chromatography. Aliphatic amines coelute as a negative peak which can be quantitated. Using a solvent of 1 mM 2-amino-5-methyl-benzenesulfonic acid, 0.05 M acetic acid, pH = 5.0 buffer and a C18 column, butylamine was quantitated. The calibration curve was linear from 1.0 M to 2.5 × 10−3M with a minimum detectable concentration of 1.01 × 10−3M with a 20 μl injection loop.