Effects of Some Fixatives on Inclusion Bodies of Infectious Bovine Rhinotracheitis.

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Abstract
Primary bovine kidney epithelial cells grown in tissue culture monolayers were infected with infectious bovine rhinotracheitis virus, treated with various common fixatives, and stained with hematoxylin and eosin. Of fixatives used, Bouin''s and Zenker''s gave the best demonstration of characteristic intranuclear inclusion bodies. Further work with components of these 2 fluids indicated that for best demonstration of inclusions, a highly acidic fixative containing acetate ions and a mordanting agent is needed.