The human muscarinic M1 acetylcholine receptor, when expressed in CHO cells, activates and downregulates both Gqα and G11α equally and non-selectively
- 14 June 1993
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 324 (2) , 241-245
- https://doi.org/10.1016/0014-5793(93)81401-k
Abstract
CHO cells express both of the phosphoinositidase C‐linked G‐proteins Gq and G11 G11α is some 2.5‐fold more highly expressed than Gqα in membranes of these cells. Following transfection and stable expression of CHO cells with DNA encoding the human muscarinic M1 acetylcholine (HM1) receptor, chronic treatment of the cells with the cholinergic agonist carbachol resulted in down‐regulation of membrane levels of both Gqα and G11α. Dose‐response curves to carbachol produced identical EC50 values for agonist‐induced down‐regulation of the two G‐proteins and both were down‐regulated with the same time course. These data indicate that the HM1 receptor interacts with and activates both Gqα and G11α equivalently and non‐selectively in a whole cell system in which the receptor has access to both G‐proteins.Keywords
This publication has 10 references indexed in Scilit:
- Activation of phosphatidylinositol lipid‐specific phospholipase C‐β3 by G‐protein βγ subunitsFEBS Letters, 1993
- Subunits βγ of heterotrimeric G protein activate β2 isoform of phospholipase CNature, 1992
- Regulation of phospholipase C by G proteinsTrends in Biochemical Sciences, 1992
- Isozyme-selective stimulation of phospholipase C-β2 by G protein βγ-subunitsNature, 1992
- Gα11 and Gαq guaninine nucleotide regulatory proteins differentially modulate the response to thyrotropin‐releasing hormone in Xenopus oocytesFEBS Letters, 1992
- Agonist control of G-protein levelsTrends in Pharmacological Sciences, 1991
- Widespread distribution of Gqα/Gllα detected immunologically by an antipeptide antiserum directed against the predicted C‐terminal decapeptideFEBS Letters, 1991
- Activation of the β1 isozyme of phospholipase C by α subunits of the Gq class of G proteinsNature, 1991
- Regulation of Polyphosphoinositide-specific Phospholipase C Activity by Purified G qScience, 1991
- G protein diversity: a distinct class of alpha subunits is present in vertebrates and invertebrates.Proceedings of the National Academy of Sciences, 1990