CHARACTERIZATION OF A RHAMNAN DERIVED FROM PREPARATIONS OF PSEUDOMONAS-AERUGINOSA LIPOPOLYSACCHARIDES

  • 1 May 1988
    • journal article
    • letter
    • Vol. 14  (5) , 701-703
Abstract
A polysaccharide isolated from the degraded lipopolysaccharides of Pseudomonas aeruginosa serogroup 07 (Lanyi .sbd.Bergan classification) was characterized by liquid chromatography, acid hydrolysis, and 1H and 13C NMR spectroscopy. It has molecular mass 15,000 and represents mainly a rhamnan of the structure .fwdarw. 2)-.alpha.-D-Rha-(1 .fwdarw. 3)-.alpha.-D-Rha-(1 .fwdarw., identical to the structure of O-specific polysaccharides of Pseudomonas aeruginosa pvs morsprunorum and cerasi. Some minor constituents, such as glucose, mannose, an unknown sugar, and phosphate, are found in the polysaccharide preparation as well. Distribution of the rhamnan in some other P. aeruginosa serogroups is discussed and its identitiy to the common polysaccharide antigen of P. aeruginosa is suggested.