The Structure of the O-Specific Chain of Lipopolysaccharide from Pseudomonas aeruginosa IID 1008 (ATCC 27584)1

Publisher Website
Google Scholar
Abstract
Structural studies were carried out on the O -polysaccharide fraction obtained from the lipopolysaccharide of Pseudomonas aeruginosa IID 1008 (ATCC 27584). The O -polysaccharide comprises L-rhamnose, N -acetyl-D-quinovosamine, N -acetyl-D galactosaminuronic acid, and N -formyl-D-galactosaminuronic acid. The characterization of oligosaccharide fragments resulting from acid hydrolysis, Smith degradation and alkaline degradation of the O -polysaccharide, together with 1 H-NMR and 13 C-NMR spectroscopic data of the polysaccharide, led to the following structure for the repeating units: →3)Rha(α1→4)GalNAcA(α1→4)GalNFoA(α1→3)QuiNAc(α1→. Almost all of the carboxyl groups of the N -acetylgalactosaminuronic acid residues and about half of the same groups of the N -formylgalactosaminuronic acid residues were in an amide form.