Genotoxicity of acrylic acid, methyl acrylate, ethyl acrylate, methyl methacrylate, and ethyl methacrylate in l5178y mouse lymphoma cells
- 1 January 1988
- journal article
- research article
- Published by Wiley in Environmental and Molecular Mutagenesis
- Vol. 11 (1) , 49-63
- https://doi.org/10.1002/em.2850110107
Abstract
A series of monomeric acrylate/methacrylate esters (methyl acrylate, ethyl acrylate, methyl methacrylate, and ethyl methacrylate) as well as acrylic acid were examined for genotoxic activity in L5178Y mouse lymphoma cells without exogenous activation. All five compounds induced concentration-dependent increases in mutant frequency. Small-colony, trifluorothymidine-resistant mutants were primarily induced, which suggests that these compounds may act via a clastogenic mechanism. This prediction was confirmed by the finding that all five compounds produced gross chromosome aberrations in mouse lymphoma cells. The two acrylates were much more potent in their response than acrylic acid. Methyl acrylate (22 μg/ml, survival = 18%) induced 385 mutants/106 survivors (total mutant frequency less the spontaneous mutant frequency) and 45 chromosome aberrations/100 cells analyzed (total aberrations less the spontaneous background). Ethyl acrylate (37.5 μg/ml, survival = 15%) induced 683 mutants/106 survivors.This publication has 39 references indexed in Scilit:
- Mutagenicity of actinomycin D in mammalian cells due to clastogenic effectsMutation Research Letters, 1987
- Mutagenicity and clastogenicity of adriamycin in L5178Y/TK+/−-3.7.2C mouse lymphoma cellsMutation Research Letters, 1987
- The isolation and characterization of 2-carboxyethyl adducts following in vitro reaction of acrylic acid with calf thymus DNA and bioassay of acrylic acid in female Hsd: (ICR)Br miceChemico-Biological Interactions, 1987
- Mutagenicity of m-AMSA and o-AMSA in mammalian cells due to clastogenic mechanism: possible role of topoisomeraseMutagenesis, 1987
- Review of the toxicity of multifunctional acrylatesJournal of Toxicology and Environmental Health, 1986
- In situ analysis of trifluorothymidine-resistant (TFTr) mutants of L5178Y/TK+/− mouse lymphoma cellsMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1985
- Chemical mutagenesis testing in Drosophila. III. Results of 48 coded compounds tested for the national toxicology programEnvironmental Mutagenesis, 1985
- The quantitation of TK−/− and HGPRT− mutants of L5178Y/TK+/− mouse lymphoma cells at varying times post‐treatmentEnvironmental Mutagenesis, 1982
- Cytogenetic analysis of the L5178Y/TK+/− → TK−/− mouse lymphoma mutagenesis assay systemMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1981
- Mutagenicity of vinyl compounds in salmonella typhimuriumTeratogenesis, Carcinogenesis, and Mutagenesis, 1981