Adenosine 3′,5′‐cyclic monophosphate (cAMP) inhibits phorbol ester‐induced growth of an IL‐2‐dependent T cell line

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Abstract
We previously established a human T cell line, TPA‐Mat, which can proliferate in response to not only interleukin‐2 (IL‐2), but also phorbol esters such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) and phorbol‐12,13‐dibutyrate (PDBu). The present study demonstrated that the PDBu‐dependent growth of TPA‐Mat cells was inhibited up to 90% by adenosine 3′,5′‐cyclic monophosphate (cAMP) raising agents such as forskolin, cholera toxin and 1‐methyl‐3‐isobutylxanthine, and cAMP analogues, whereas the IL‐2‐stimulated TPA‐Mat growth was slightly inhibited. These findings suggest that the signal transduction pathway of PDBu‐induced growth, which should involve activation of protein kinase C, is sensitive to cAMP, and that it cannot be exactly identical to the signal transduction pathway of Il‐2‐induced growth in TPA‐Mat cells.