PREPARATION AND PROPERTIES OF CELL-FREE “FORMIC HYDROGENLYASE” FROM ESCHERICHIA COLI

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Abstract
The enzymatic system is markedly inhibited by the metal-complexing agent,[alpha],[alpha],''-dipy-ridyl, and the inhibition is most effectively relieved by Fe++. In contrast to the activity of "hydrogenlyase" the activity of hydrogenase in the cell-free extract with fumarate as the acceptor is relatively insensitive to a,a''-dipyridyl.