PREVENTION OF INSULIN-I131ADSORPTION TO GLASS

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Abstract
It has been shown that human serum albumin in a final concentration of 0.5 mg./ml. can effectively prevent insulin-I131 from being adsorbed to glass surfaces. Of the insulin-I131 introduced to the system 95.8% was recoverable when precipitated with 20% trichloroacetic acid after a period of incubation for 30 minutes when protected by this concentration of albumin. It appears that human serum albumin can be reliably employed to protect insulin-I131 when dilutions of standards or working solutions are made for radioactivity measurements.

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