Characterization of double-stranded ribonucleic acid sequences present in the initial transcription products of rat liver chromatin

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Abstract
At low ionic strength and with a low exogenous RNA polymerase/DNA ratio, rat liver chromatin directed the synthesis in vitro of RNA sequences rich in double-stranded segments. All the transcripts contained at least 1 double-stranded sequence. Most of the double-stranded segments are formed by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. They are heterogeneous in size, 35-45% of them being more than 80 nucleotides long. They contain 61-64% G + C, whether synthesized by rat liver RNA polymerase (form B) or Escherichia coli RNA polymerase. The largest double-stranded sequences were found in the largest transcripts, and are the most thermostable. The fidelity of base-matching is better in double-stranded transcripts synthesized on rat liver chromatin by homologous polymerase than in those synthesized on it by a bacterial polymerase, or in those synthesized by either of the 2 polymerases on pure DNA.

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