A mutant LexA repressor harboring a cleavage motif cysteine‐glycine remains inducible
- 25 April 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 231 (2) , 437-439
- https://doi.org/10.1016/0014-5793(88)80866-4
Abstract
Using site-directed mutagenesis of the lexA gene we have changed the amino acid Ala-84 of the LexA repressor for a cysteine. The reason for this change was the striking homology between LexA and UmuD and the comparable size of the two amino acid side chains. Using an in vivo repression/induction assay it is shown that the LexA-Cys-84 mutant remains inducible by mitomycin C and UV irradiation essentially in the same way as the wild-type repressorKeywords
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