Purification and Some Properties of Two Viruses Infecting the Cultivated Mushroom Agaricus bisporus
- 1 February 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 42 (2) , 231-240
- https://doi.org/10.1099/0022-1317-42-2-231
Abstract
Highly purified preparations of the isometric mushroom viruses [MV] 1 and 4 were made by a combination of differential centrifugation, ion-exchange chromatography on DEAE-cellulose and sucrose density-gradient centrifugation. Purified preparations of MV1 virions (25 nm diameter) sedimented at 130S and contained 1 capsid polypeptide of MW 24,400 and 2 species of double-stranded RNA of MW about 1.4 .times. 106. MV4 particles (35 nm diameter) had a sedimentation value of 140-145S and contained 1 capsid polypeptide of MW 63,800 and 2 species of double-stranded RNA of MW 1.5 .times. 106 and 1.4 .times. 106. MV1 and MV4 were antigenically unrelated.This publication has 1 reference indexed in Scilit:
- An Examination of Permeation Chromatography on Columns of Controlled Pore Glass for Routine Purification of Plant VirusesJournal of General Virology, 1977