NADH- and NAD(P)H-Nitrate Reductases in Rice Seedlings
- 1 September 1976
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 58 (3) , 292-294
- https://doi.org/10.1104/pp.58.3.292
Abstract
By use of affinity chromatography on blue dextran-Sepharose NADH:nitrate oxidoreductase (EC 1.6.6.1) and NAD(P)H:nitrate oxidoreductase (EC 1.6.6.2) from rice (Oryza sativa L.) were partially separted. Nitrate-induced seedlings contained more NADH-nitrate reductase than NAD(P)H-nitrate reductase, whereas chloramphenicol-induced seedlings contained primarily NAD(P)H-nitrate reductase. NAD(P)H-nitrate reductase utilized NADPH directly as reductant. This enzyme has a preference for NADPH, but reacts about half as well with NADH.This publication has 7 references indexed in Scilit:
- Purification of NADH-Nitrate Reductase by Affinity ChromatographyPlant Physiology, 1975
- Specificity for Nicotinamide Adenine Dinucleotide by Nitrate Reductase from LeavesPlant Physiology, 1974
- Inhibitor of nitrate reductase in the roots of rice seedlings and its effect on the enzyme activity in the presence of NADHBiochimica et Biophysica Acta (BBA) - Enzymology, 1974
- Rapid purification of lactate dehydrogenase from rat liver and hepatoma: A new approachArchives of Biochemistry and Biophysics, 1974
- Nitrate Reductase of Rice Seedlings and Its Induction by Organic Nitro-CompoundsPlant Physiology, 1972
- Structural and functional relationships of enzyme activities induced by nitrate in barleyBiochemical Journal, 1970
- The Induction of Nitrate Reductase and the Preferential Assimilation of Ammonium in Germinating Rice SeedlingsPlant Physiology, 1969