PHOTOREACTIVATING ENZYME FROM STREPTOMYCES GRISEUS—III. EVIDENCE FOR THE PRESENCE OF AN INTRINSIC CHROMOPHORE

Publisher Website
Google Scholar
Abstract
—: Evidence is presented that DNA photoreactivating enzyme from Streptomyces griseus consists of a high molecular protein part and a low molecular chromophore which is released by denaturation. The free chromophore is highly fluorescent and has an absorption maximum at 420 nm. In native photoreactivating enzyme the chromophore fluorescence is almost completely quenched and there is an additional absorption band at 445 nm. Native photoreactivating enzyme spontaneously looses its chromophore following first order kinetics as measured by the increase of fluorescence intensity. A good correlation was found between the increase of fluorescence intensity and the decrease of biological activity, stressing the importance of the chromophore‐protein bond. The presence of DNA greatly retards the spontaneous release of chromophore, and with UV‐irradiated DNA the photoreactivating enzyme is almost completely stable. In five different chromatographic systems, cochromatography of biological activity and enzyme‐bound chromophore was found, thus ruling out the possibility that the observed chromophore belongs to a contamination in the enzyme preparation. Photoreactivating enzyme binds very strongly to Blue‐Sepharose indicating the presence of a positive charge in the polynucleotide binding site.