Isolation of the DNA of the E. coli chromosome in one piece.
- 1 April 1966
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 55 (4) , 792-797
- https://doi.org/10.1073/pnas.55.4.792
Abstract
A method is described for extracting the DNA from E. coli by lysing spheroplasts on top of a Csd solution by the combined action of pronase and sodium dodecyl sarcosinate. Density gradient analysis of DNA isolated by this method from cells that have incorporated 5-bromouracil into their DNA for about one third of a replication cycle reveals that the entire DNA complement of the chromosome can be isolated as one molecule.This publication has 14 references indexed in Scilit:
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