ALLOSPECIFIC T-CELL LINES WITH FUNCTIONAL ACTIVITIES

  • 1 January 1982
    • journal article
    • research article
    • Vol. 46  (2) , 281-287
Abstract
A mouse T lymphocyte line and sublines from it reactive with Class II major histocompatibility complex (MHC) alloantigens were examined for proliferative responses and helper activity in vitro and in vivo. Both the parent cell line and its derived sublines recognize alloantigenic determinants encoded for by the I-A subregion of the H-2 complex in a proliferation assay, and have regulatory activity for allogeneic and syngeneic B cells in 2 different helper systems. The vitro selected cell populations are .apprx. 20-fold more active in functional helper assays than populations selected in vivo by priming. Differential analysis of the patterns of proliferative responses of sublines against allogeneic I-A molecules on different backgrounds indicates that 1 type of selected cells reacts preferentially to MHC molecules presented on the background used for selection.