Rapid and Specific Isolation of Radioactive Glucose from Biological Samples

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Abstract
An easy, reliable and specific ion-exchange method is presented for isolating glucose for specific radioactivity determinations from both blood plasma [for measuring gluconeogenesis in livestock] and buffered in vitro incubation media. The use of a glucose binding resin (borate-charged anion resin) combines speed of ion exchange with specificity of derivative formation. Glucose specific radioactivities, determined by ion exchange on protein-free filtrates of plasma containing [14C]-glucose, show excellent agreement with those from the popular glucose pentaacetate derivative method and are less viable. Carry-over of labeled acetate, propionate, lactate, glyoxylate, alanine, aspartate or glutamate into the glucose fraction is < 0.2%. Glycerol carry-over is 1.2%. Glucose recovery is increased .apprx. 3 times that of the glucose pentaacetate derivative method and averaged 94% from plasma filtrates.