High Affinity scFv−Hapten Pair as a Tool for Quantum Dot Labeling and Tracking of Single Proteins in Live Cells
- 20 November 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Nano Letters
- Vol. 8 (12) , 4618-4623
- https://doi.org/10.1021/nl8032284
Abstract
We describe a general approach to label cell surface proteins using quantum dots (QD) for single-molecule tracking. QDs coated with small-hapten modified peptides are targeted to cell surface fusion proteins containing the corresponding single-chain fragment antibody (scFv). The approach is illustrated with the small hapten fluorescein (FL) and a high-affinity anti-FL scFv fused to two different proteins in yeast and murine neuronal cell line N2a.This publication has 17 references indexed in Scilit:
- Advances in Single-Molecule Fluorescence Methods for Molecular BiologyAnnual Review of Biochemistry, 2008
- Solubilization of Quantum Dots with a Recombinant Peptide from Escherichia coliSmall, 2007
- Site-specific protein modification: advances and applicationsCurrent Opinion in Chemical Biology, 2007
- Isolation and affinity maturation of hapten-specific antibodiesBiotechnology Advances, 2007
- The Fluorescent Toolbox for Assessing Protein Location and FunctionScience, 2006
- Site-specific labeling of proteins with small molecules in live cellsCurrent Opinion in Biotechnology, 2005
- Quantum Dots for Live Cells, in Vivo Imaging, and DiagnosticsScience, 2005
- Bioactivation and Cell Targeting of Semiconductor CdSe/ZnS Nanocrystals with Phytochelatin-Related PeptidesJournal of the American Chemical Society, 2004
- Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot TrackingScience, 2003
- Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinityProceedings of the National Academy of Sciences, 2000