Hydrolysis of cellulose using ternary mixtures of purified celluloses

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Abstract
The saccharification of microcrystalline cellulose by reconstituted ternary mixtures of purified cellulases (one endoglucanase and two cellobiohydrolases) has been studied over the entire range of mixture compositions. Ternary plots are used to compare the performance of five synthetic mixtures drawn from the cellulase systems ofAcidothermus cellulolyticus, Trichoderma reesei, Thermomonospora fusca, andThermotoga neapolitana. Results reveal that at least one synthetic mixture utilizing enzymes from three different organisms delivers performance competitive with that of a “native” (i.e., co-evolved) ternary system drawn exclusively fromT. reesei. This heterologous system, consisting of the endoglucanase El fromA. cellulolyticus and the exoglucanases CBHI fromT. ressei and E3 fromT. fusca, is forgiving from the system-design point of view, in that it delivers high saccharification rates over a wide range of mixture compositions.

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