Scanning Electron Microscopy Of Uncoated Human Metaphase Chromosomes
- 1 March 1979
- journal article
- research article
- Published by Wiley in Journal of Microscopy
- Vol. 115 (2) , 151-160
- https://doi.org/10.1111/j.1365-2818.1979.tb00164.x
Abstract
Human metaphase chromosomes were processed with a 3% glutaraldehyde-tannic acid technique and examined in a scanning electron microscope at 20 kV either without added metal coating or with 2 nm of sputtered gold coating. Several substrates—aluminium foil, silver mirror deposit and sputtered gold—provided good conductive backgrounds for chromosomal spreads. Silver mirror deposit was the best conductive substrate tested. This method should prove to be a useful tool for monitoring the three-dimensional morphology of mitotic chromosomes with the possibility of studying various banding techniques, chromosomal uncoiling and secondary constrictions currently being examined in chromosomal studies.This publication has 8 references indexed in Scilit:
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