Cloning of chicken lysozyme structural gene sequences synthesized in vitro
Open Access
- 1 September 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 5 (9) , 3275-3294
- https://doi.org/10.1093/nar/5.9.3275
Abstract
Double-stranded chicken lysozyme cDNA was synthesized from an oviduct' mRNA fraction enriched for lysozyme mRNA. The ds-cDNA was inserted into the Bam HI site of plasmid pBR322 using chemically synthesized DNA linker molecules containing the Bam HI restriction endonuclease cleavage site. After bacterial transformation, colonies carrying lysozyme DNA were identified by hybridization with highly purified lysozyme cDNA. The 555 base pairs long cloned DNA fragment of one recombinant plasmid was isolated and characterized by restriction endonuclease digestion. The DNA sequence of selected parts of the inserted DNA is as predicted from the amino acid sequence of prelysozyme. The sequence data allows the unambiguous location of the coding region within lysozyme mRNA.Keywords
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