Inhibition by kinase inhibitors of the development and the disappearance of the preprophase band of microtubules in tobacco BY-2 cells
Open Access
- 1 October 1992
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 103 (2) , 397-405
- https://doi.org/10.1242/jcs.103.2.397
Abstract
The effects of two kinase inhibitors, staurosporine and K-252a, on the development and the disappearance of the preprophase band of microtubules (PPB) were studied in tobacco BY-2 cells, the cell cycle of which had been synchronized. Treatment of cells at late G2 phase or at prophase with 20 M staurosporine or 2 M K-252a for 2 or 3 h caused a decrease in the rate of development of PPBs and inhibited the disappearance of PPBs once they had developed, with the resultant accumulation of cells with a PPB. Cortical microtubules (MTs) or PPBs on protoplast ghosts, which were prepared from BY-2 cells in late G2 phase or at prophase, disappeared on treatment of the ghosts with ATP or ATP S but not on treatment with AMP-PNP. The effect of ATP on the disappearance of MTs on the ghosts was suppressed by 20 M staurosporine. Although 2 M K-252a applied to the culture medium suppressed the anticipated disappearance of PPBs, it did not suppress the disappearance of MTs on the ghosts that was caused by ATP. We propose tentatively that a kinase sensitive to staurosporine but not to K-252a is directly involved in the disappearance of MTs and that a kinase that is sensitive to K-252a is indirectly involved in this process. The disappearance of MTs on protoplast ghost that was induced by ATP did not occur in the presence of 20 μM taxol.Keywords
This publication has 22 references indexed in Scilit:
- Uncoupled cell cycle without mitosis induced by a protein kinase inhibitor, K-252a.The Journal of cell biology, 1991
- The plant cytoskeletonCurrent Opinion in Cell Biology, 1991
- cDNA cloning of a novel cdc2+/CDC28‐related protein kinase from riceFEBS Letters, 1991
- Highly synchronous culture of fibroblasts from G2 block caused by staurosporine, a potent inhibitor of protein kinasesExperimental Cell Research, 1991
- Rearrangements of Microtubules Involved in Establishing Cell Division Planes Start Immediately after DNA Synthesis and Are Completed Just before MitosisPlant Cell, 1990
- Universal control mechanism regulating onset of M-phaseNature, 1990
- Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggsNature, 1990
- Cytoskeletal Ultrastructure of Phragmoplast-Nuclei Complexes Isolated from Cultured Tobacco CellsPublished by Springer Nature ,1988
- Staurosporine, a potent inhibitor of phospholipidCa++dependent protein kinaseBiochemical and Biophysical Research Communications, 1986
- K-252a, a potent inhibitor of protein kinase C from microbial origin.The Journal of Antibiotics, 1986