Surfaces and Orientations: Much to FRET about?
- 27 January 2005
- journal article
- review article
- Published by American Chemical Society (ACS) in Accounts of Chemical Research
- Vol. 38 (7) , 542-548
- https://doi.org/10.1021/ar040138c
Abstract
Single molecule FRET (fluorescence resonance energy transfer) is a powerful technique for detecting real-time conformational changes and molecular interactions during biological reactions. In this Account, we examine different techniques of extending observation times via immobilization and illustrate how useful biological information can be obtained from single molecule FRET time trajectories with or without absolute distance information.Keywords
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This publication has 45 references indexed in Scilit:
- Single-molecule imaging of cooperative assembly of -hemolysin on erythrocyte membranesThe EMBO Journal, 2003
- Methods of single-molecule fluorescence spectroscopy and microscopyReview of Scientific Instruments, 2003
- RATIOMETRICSINGLE-MOLECULESTUDIES OFFREELYDIFFUSINGBIOMOLECULESAnnual Review of Physical Chemistry, 2001
- Dynamics and folding of single two-stranded coiled-coil peptides studied by fluorescent energy transfer confocal microscopyProceedings of the National Academy of Sciences, 2000
- Measuring conformational dynamics of biomolecules by single molecule fluorescence spectroscopy.Nature Structural & Molecular Biology, 2000
- The renaissance of fluorescence resonance energy transfer.Nature Structural & Molecular Biology, 2000
- Single-molecule protein folding: Diffusion fluorescence resonance energy transfer studies of the denaturation of chymotrypsin inhibitor 2Proceedings of the National Academy of Sciences, 2000
- Single-molecule imaging of EGFR signalling on the surface of living cellsNature Cell Biology, 2000
- Fluorescence Spectroscopy of Single BiomoleculesScience, 1999
- Energy transfer: a spectroscopic ruler.Proceedings of the National Academy of Sciences, 1967