Incorporation of calliphorin into the cuticle of the developing blowfly, Calliphora vicina
- 1 July 1986
- journal article
- research article
- Published by Springer Nature in Wilhelm Roux' Archiv für Entwicklungsmechanik der Organismen
- Vol. 195 (5) , 296-301
- https://doi.org/10.1007/bf00376062
Abstract
The stage-specific appearance of calliphorin in cuticles of Calliphora vicina was analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. The fate of the protein, injected into last instar larvae, was pursued by autoradiography of histological sections. Fractionation of sclerotized pupal cuticle in buffer-soluble, urea-soluble and NaOH-soluble fractions shows that calliphorin forms covalent and non-covalent links with other cuticle components. Calliphorin traverses the epidermal cells and enters the cuticle in an undegraded state and appears to be an important constituent of the sclerotizing system.Keywords
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