Some Immunological Investigations on Antithrombin III ‘Budapest’

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Abstract
Immunoelectrophoretic studies were carried out on plasma from a patient with an abnormal antithrombin III molecule (antithrombin III ''Budapest''). One-dimensional immunoelectrophoresis using a commercial antibody [Ab] to antithrombin III (Ab to .alpha.2-AtIII) showed 2 precipitin peaks of .alpha.2-AtIII antigen [Ag]. Two-dimensional immunoelectrophoresis showed 2 precipitin peaks, 1 with normal electrophoretic mobility, the other with increased electrophoretic mobility. .alpha.2-AtIII Ag with normal mobility was identical to normal .alpha.2-AtIII and the .alpha.2-AtIII Ag with increased electrophoretic mobility was antigenically deficient and appeared to be present in higher concentration than the normal .alpha.2-AtIII Ag. Although 1-dimensional immunoelectrophoresis on plasma from the patient''s son showed only 1 peak, 2-dimensional immunoelectrophoresis revealed that the son had 2 populations of .alpha.2-AtIII, 1 with normal mobility, the other with increased mobility. The .alpha.2-AtIII Ag with normal mobility appeared to be present in higher concentration than the .alpha.2-AtIII Ag with increased mobility. One- and 2-dimensional immunoelectrophoresis using a different commerical Ab to .alpha.2-AtIII showed only 1 precipitin peak using the patient''s plasma. The precipitin peak observed following 2-dimensional immunoelectrophoresis was asymmetric and showed increased mobility.

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