Nonblinking and long-lasting single-molecule fluorescence imaging
Top Cited Papers
- 1 October 2006
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 3 (11) , 891-893
- https://doi.org/10.1038/nmeth934
Abstract
Photobleaching and blinking of fluorophores pose fundamental limitations on the information content of single-molecule fluorescence measurements. Photoinduced blinking of Cy5 has hampered many previous investigations using this popular fluorophore. Here we show that Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.Keywords
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