EMPLOI D'UN MILIEU SYNTHÉTIQUE SIMPLIFIÉ POUR LA CULTURE IN VITRO DE CELLULES ANIMA LES ET DU VIRUS DE LA POLIOMYÉLITE

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Abstract
The nutritive medium SN-3 is composed of Hanks''s balanced salt solution with ribose, glutamine, and cysteine added; the glucose concentration is doubled. This nutritive solution, without additional ingredients, can keep alive monkey or mouse kidney tissue cultures during a period of several weeks. When this medium is supplemented with serum, it stimulates the in vitro cellular proliferation of freshly trypsinized monkey kidney tissue and supports the growth of He La cells. We have used this simplified synthetic medium for the preparation of experimental lots of poliomyelitis vaccine. The virus concentration of the suspension of the three virus strains, Mahoney, MEF-1, and Saukett, produced with this new medium has been found as high as the concentration obtained with the more complex nutritive media usually used. We did not observe any important differences between the vaccine prepared with the SN-3 medium and that prepared with the usual media.