High-Performance Liquid Chromatographic Method for the Determination of Metoclopramide in Plasma

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Abstract
A reversed-phase high performance liquid chromatographic method for the determination of metoclopramide in plasma has been developed. Metoclopramide was extracted from alkalinized plasma (0.25 − 0.5 ml) into methyl-t-butyl ether, injected onto an alkylnitrile column and eluted with a mobile phase of methanol and tetrahydrofuran in acetate buffer, pH 4.3. The eluate was monitored with a variable wavelength UV detector at 309 nm. Extraction efficiencies for metoclopramide and the internal standard (a dipropyl analog of procainamide) ranged from 89 to 93%. The limit of sensitivity for the method was 2.5 ng/ml when 0.5 ml of plasma was extracted. Application of the method to single dose pharmacokinetic studies was demonstrated by the analysis of plasma samples following the administration of a single 10 mg oral dose of metoclopramide hydrochloride to two healthy volunteers.

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