A New Principle for Large Scale Production of Insulin.

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Abstract
The conventional method for large scale manufacture of insulin starts with an extraction of the pancreatic glands with acidified ethyl alcohol and subsequent evaporation of the alcohol in vacuo at low temperature. The authors present a technique of adsorbing the insulin from the alcoholic extract at pH about 3 on an ion exchanger, alginic acid, with subsequent elution of the insulin by means of 0.2 N HC1. The use of a cumbersome and expensive distilling equipment is thereby made superfluous. Fats are not adsorbed. An increased yield of insulin is obtained if the traces of tryptic enzymes still present in the extract are destroyed by heating the alcoholic extract at pH 3.2 to 70[degree]C before adsorbtion.

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