Analysis of the Haemolymph of Arion Ater L. (Gastropoda: Pulmonata)

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Abstract
Methods are described for the collection of slug haemolymph and its analysis for cations, anions, total electrolytes, protein, Δ and pH. Of the cations measured, potassium and calcium show the greatest variation, being two to three times as variable as sodium, the most constant. About 20 % of the blood calcium exists in a bound form. Of the anions measured, chloride is the most concentrated and least variable. Sulphate and alkalinity are about equally variable, three times the chloride fluctuation. The phosphate concentration is very low. The variation of total electrolytes is only half that of the total solutes, as measured by Δ, while protein is very variable. The pH is remarkably alkaline but varies only within narrow limits. Formulae are given for two physiological salines, capable of maintaining both muscular and ciliary activity.