LIGHT enhances the bactericidal activity of human monocytes and neutrophils via HVEM

Abstract
Human monocytes and neutrophils play major roles in clearing bacteria from human blood and tissues. We found that the herpes virus entry mediator (HVEM) was highly expressed in monocytes and neutrophils, and its interaction with “homologous to lymphotoxins, shows inducible expression, and competes with herpes simplex virus glycoprotein D for HVEM/tumor necrosis factor (TNF)‐related 2” (LIGHT) enhanced bactericidal activity against Listeria monocytogenes and Staphylococcus aureus. The LIGHT‐HVEM interaction increased levels of phagocytosis, interleukin (IL)‐8, TNF‐α, nitric oxide (NO), and reactive oxygen species (ROS) in monocytes and neutrophils. Anti‐HVEM monoclonal antibody was able to block LIGHT‐induced bactericidal activity, cytokine production (IL‐8 and TNF‐α), and ROS generation. Moreover, inhibition of ROS and NO production blocked LIGHT‐induced bactericidal activity. Our results indicate that the LIGHT/HVEM interaction in monocytes and neutrophils contributes to antibacterial activity.
Funding Information
  • SRC fund to the IRC at the University of Ulsan from Kosef
  • Ministry of Korean Science and Technology

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