INVITRO PROLIFERATION OF HEMATOPOIETIC CELLS IN THE PRESENCE OF ADHERENT CELL-LAYERS .2. DIFFERENTIAL EFFECT OF ADHERENT CELL-LAYERS DERIVED FROM VARIOUS ORGANS

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  • 1 January 1979
    • journal article
    • research article
    • Vol. 7  (1) , 52-58
Abstract
Mouse bone marrow-derived adherent cell populations promoted proliferation of hemopoietic cells in vitro in a liquid culture system for at least 4 wk. Adherent cell layers derived from other hemopoietic organs (fetal liver, adult spleen) and fibroblasts from embryonic tissues did not maintain hemopoietic cells in this system. Medium, conditioned by adherent cells derived from fetal liver, spleen and embryonic tissues, displayed a considerable colony stimulating activity (CSA). Marrow-derived adherent cells produced no detectable CSA. A possible relationship between the in vitro expression of a growth-promoting activity of an adherent cell population on hemopoietic cells and its endogenous CSA production, was discussed.