On the origin of the multiple forms of rabbit γ-globulin

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Abstract
The rate of incorporation of radioactive amino acids into different chromatographic fractions of rabbit [gamma] -globulin was investigated. It was shown by experiments in vivo that there is no evidence for conversion of one form of [gamma] -globulin into another, or of [gamma] -globulin into antibody, in the vascular system. Experiments in vitro showed that amino acids are incorporated into different fractions of [gamma] -globulin at different rates, with an obvious difference between the spleen and the various lymph nodes and bone marrow which were tested. It is suggested that different types of [gamma] -globulin are synthesized by different cells which are unevenly distributed in the tissues making [gamma] -globulin. The observation that different tissues synthesize different fractions of [gamma] -globulin at different rates may in turn explain the earlier observation that chromatographically different antibodies appear at successive stages in the immunization of a rabbit.