Nucleotide Sequence of cDNA Clones Encoding the Outer Capsid Protein, VP5, of Bluetongue Virus Serotype 10
- 1 May 1986
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 67 (5) , 957-962
- https://doi.org/10.1099/0022-1317-67-5-957
Abstract
Summary The complete sequence of the RNA segment that codes for a major outer capsid protein (VP5) of bluetongue virus serotype 10 has been determined from overlapping cDNA clones inserted into pBR322. The segment 5 RNA of the virus (M5 RNA) is deduced to be 1638 base pairs long (1.05 × 106 daltons) and has an open reading frame in one strand capable of coding for a protein with a calculated size of 59163 daltons (526 amino acids) and a net charge of -4.5 at neutral pH.Keywords
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