Influence of Yolk-Citrate, Yolk-Glycine, Whole Milk, and Skimmilk Extenders Containing Glycerol on Motility of Bovine Sperm at 37 and 5° C.

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Abstract
Sixteen samples were extended 150 in 14 extenders with 7.5% glycerol added by direct and step-wise procedures to give 28 different extender treatments. Aliquots of each treatment were stored at 37t for 12 hr. and at 5[degree]C for 1 week. Yolk-2.9% citrate, yolk-2.0% citrate, yolk-2.9% glycine, whole milk, and skimmilk were the basic extenders, together with mixtures of these at 1:1 in all possible pair combinations. Direct glycerolation generally gave better results than stepwise, as evaluated by progressive sperm motility, but these effects were not significant by 7 days of storage at 5[degree]C. Independent of glycerolation method, progressive motility at 37[degree]C was significantly higher in yolk-2.9% citrate and its combinations than in yolk-2.0% citrate and its combinations, but the reverse was true at 5[degree]C. Extenders composed solely of milk constituents performed favorably at 5[degree]C and poorly with prolonged 37[degree]C storage. No single extender or combination of 2 basic extenders gave similar results, as time and temperature of storage and method of adding glycerol were varied.